Journal: International Journal of Molecular Sciences

Article Title: Role and Regulation of Mechanotransductive HIF-1α Stabilisation in Periodontal Ligament Fibroblasts

doi: 10.3390/ijms21249530

Figure Lengend Snippet: Effects of compressive strain with and without treatment with glycosaminoglycan antagonist surfen on p-ERK ( a ), ERK ( b ), PTK-2 ( c ), HIF-1α ( d ), PTGS-2 /PG-E2 expression ( e , f ), and VEGF expression ( g , h ). Protein expression: n = 6; gene expression: n = 9. Statistics: ordinary one-way ANOVA followed by Holm–Sidak’s multiple comparison tests or Welch-corrected ANOVA followed by Games–Howell multiple comparison tests; AU = arbitrary units; * p < 0.05, ** p < 0.001, *** p < 0.001.

Article Snippet: To investigate mechanotransductive integrin signalling , PTK-2 inhibitor genistein (G6649) and glycosaminoglycan antagonist surfen (S6951) were obtained from Sigma-Aldrich (St. Louis, MO, USA) and used in a concentration of 5 µM for the experiments.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Role and Regulation of Mechanotransductive HIF-1α Stabilisation in Periodontal Ligament Fibroblasts

doi: 10.3390/ijms21249530

Figure Lengend Snippet: Effects of compressive strain with and without PTK-2 inhibitor genistein on p-ERK ( a ), ERK ( b ), PTK-2 ( c ), HIF-1α ( d ), PTGS-2 /PG-E2 expression ( e , f ), and VEGF expression ( g , h ). Protein expression: n = 6; gene expression: n = 15. Statistics: ordinary one-way ANOVA followed by Holm–Sidak’s multiple comparison tests or Welch-corrected ANOVA followed by Games–Howell multiple comparison tests; AU = arbitrary units; * p < 0.05, ** p < 0.001, *** p < 0.001.

Article Snippet: To investigate mechanotransductive integrin signalling , PTK-2 inhibitor genistein (G6649) and glycosaminoglycan antagonist surfen (S6951) were obtained from Sigma-Aldrich (St. Louis, MO, USA) and used in a concentration of 5 µM for the experiments.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Role and Regulation of Mechanotransductive HIF-1α Stabilisation in Periodontal Ligament Fibroblasts

doi: 10.3390/ijms21249530

Figure Lengend Snippet: Simplified schematic presentation of a possible role of heparane sulfate integrin interaction and downstream phosphorylation of kinases for the mechanotransductive stabilisation of HIF-1α. To address this question, glycosaminoglycan antagonist surfen and PTK-2 inhibitor genistein were applied. αβ: integrins, PTK2: protein tyrosine kinase 2, p-ERK: phosphorylated extracellular signal-regulated kinases.

Article Snippet: To investigate mechanotransductive integrin signalling , PTK-2 inhibitor genistein (G6649) and glycosaminoglycan antagonist surfen (S6951) were obtained from Sigma-Aldrich (St. Louis, MO, USA) and used in a concentration of 5 µM for the experiments.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Role and Regulation of Mechanotransductive HIF-1α Stabilisation in Periodontal Ligament Fibroblasts

doi: 10.3390/ijms21249530

Figure Lengend Snippet: Effects of compressive strain with and without treatment with glycosaminoglycan antagonist surfen on p-ERK ( a ), ERK ( b ), PTK-2 ( c ), HIF-1α ( d ), PTGS-2 /PG-E2 expression ( e , f ), and VEGF expression ( g , h ). Protein expression: n = 6; gene expression: n = 9. Statistics: ordinary one-way ANOVA followed by Holm–Sidak’s multiple comparison tests or Welch-corrected ANOVA followed by Games–Howell multiple comparison tests; AU = arbitrary units; * p < 0.05, ** p < 0.001, *** p < 0.001.

Article Snippet: After blocking the membrane with 5% milk (T145.3, Carl Roth) in PBS-T, the membrane was incubated with the primary antibodies anti-HIF-1α (10006421, Cayman chemicals, Ann Arbor, MI, USA, diluted 1:1000), anti-p-ERK (4370, Cell Signaling, Danvors, MA, USA, diluted 1:1000), anti-ERK (9102, Cell Signaling, Danvors, MA, USA, diluted 1:1000), anti-PTK-2 (ABIN4901515, antibodies-online, Aachen, Germany, diluted 1:1000) and anti-ACTIN (E1C602, Enogene, New York, NY, USA, diluted 1:5000) overnight at 4 °C.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Role and Regulation of Mechanotransductive HIF-1α Stabilisation in Periodontal Ligament Fibroblasts

doi: 10.3390/ijms21249530

Figure Lengend Snippet: Effects of compressive strain with and without PTK-2 inhibitor genistein on p-ERK ( a ), ERK ( b ), PTK-2 ( c ), HIF-1α ( d ), PTGS-2 /PG-E2 expression ( e , f ), and VEGF expression ( g , h ). Protein expression: n = 6; gene expression: n = 15. Statistics: ordinary one-way ANOVA followed by Holm–Sidak’s multiple comparison tests or Welch-corrected ANOVA followed by Games–Howell multiple comparison tests; AU = arbitrary units; * p < 0.05, ** p < 0.001, *** p < 0.001.

Article Snippet: After blocking the membrane with 5% milk (T145.3, Carl Roth) in PBS-T, the membrane was incubated with the primary antibodies anti-HIF-1α (10006421, Cayman chemicals, Ann Arbor, MI, USA, diluted 1:1000), anti-p-ERK (4370, Cell Signaling, Danvors, MA, USA, diluted 1:1000), anti-ERK (9102, Cell Signaling, Danvors, MA, USA, diluted 1:1000), anti-PTK-2 (ABIN4901515, antibodies-online, Aachen, Germany, diluted 1:1000) and anti-ACTIN (E1C602, Enogene, New York, NY, USA, diluted 1:5000) overnight at 4 °C.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Role and Regulation of Mechanotransductive HIF-1α Stabilisation in Periodontal Ligament Fibroblasts

doi: 10.3390/ijms21249530

Figure Lengend Snippet: Simplified schematic presentation of a possible role of heparane sulfate integrin interaction and downstream phosphorylation of kinases for the mechanotransductive stabilisation of HIF-1α. To address this question, glycosaminoglycan antagonist surfen and PTK-2 inhibitor genistein were applied. αβ: integrins, PTK2: protein tyrosine kinase 2, p-ERK: phosphorylated extracellular signal-regulated kinases.

Article Snippet: After blocking the membrane with 5% milk (T145.3, Carl Roth) in PBS-T, the membrane was incubated with the primary antibodies anti-HIF-1α (10006421, Cayman chemicals, Ann Arbor, MI, USA, diluted 1:1000), anti-p-ERK (4370, Cell Signaling, Danvors, MA, USA, diluted 1:1000), anti-ERK (9102, Cell Signaling, Danvors, MA, USA, diluted 1:1000), anti-PTK-2 (ABIN4901515, antibodies-online, Aachen, Germany, diluted 1:1000) and anti-ACTIN (E1C602, Enogene, New York, NY, USA, diluted 1:5000) overnight at 4 °C.

Techniques: